Production of enzyme tannase by solid state fermentation. Production, characterization of tannase from penicillium. In ssf with jamun leaves, the maximum production of tannase was found to be at 30 degrees c after 96 h of incubation. Demain al, solomon na eds manual of industrial microbiology and biotechnology. Solid state fermentation offers a number of economic advantages over conventional submerged fermentation for enzyme production mudgett, 1986. Improved production of tannase by klebsiella pneumoniae. Solid state fermentation involves nonaseptic conditions with the use. The ssf is alternative to submerged fermentation for production of value added products like. This is the first report on production of tannase by aspergillus.
Enzyme production was studied under solid state fermentation using different tannin. Production of tannase by aspergillus niger aa20 in. Lonsane, in advances in applied microbiology, 1997. To compare the yield of tannase by pure and coculture, to enhance the yield of tannase by using carbon glucose. Production of tannase from aspergillus niger under solid state fermentation zainab saadi mahdi, shatha salman altahan department of biology, college of science, university of baghdad, baghdad, iraq abstract thirteen a. The results indicated that the unpasteurized method before fermentation was in favor of the higher enzymic activity than the sterilized method. In the present study 31 species of penicillium isolated from caatinga and atlantic forest were tested quantitatively for the production of tannase.
Guava juice is known to be rich in antioxidant activity due a high level of vitamins a and c. Statistical optimization for tannase production by. Production of tannase under solidstate fermentation and. Initially maize, rice, wheat, barley, wheat bran were compared as carrier.
Production of tannase by aspergillus species ucp1284 was studied using solid state fermentation and cashew bagasse as substrate. This study analyzed the production of tannase by aspergillus sp. The solid state fermentation is most commonly carried out for the production of fermented food products such as bread, fermented fish, meat, yogurt, cheese and pickles. The ability of aspergillus oryzaefor the production of tannase by solid state fermentation was investigated using cashew apple bagasse cab as substrate. Substantial quantities of the enzyme were synthesized throughout the operating periods and imperfect steady. Tannase production by solid state fermentation of cashew apple. Perspectives of solid state fermentation for production of. Enzyme puri cation was achieved by a combination of concentration by rotary vacuum evaporator followed by ammonium sulphate precipitationand chromatographicresolutionusing deaecellulose column. Pdf production of tannase from aspergillus ruber under. The substrate used for the production of tannase was tamarind seed powder a similar work was done by sabu et al. Tap water was found to be the best moistening agent, with ph 5. Optimization of culture conditions for tannase production.
Solidstate fermentation an overview sciencedirect topics. Tannase production by aspergillus fumigatus ma under solid. Tannase production by penicillium purpurogenum paf6 in solid state fermentation of tanninrich plant residues following ovat and rsm. Promising substrates to increase the production of tannase. The fungal culture produced high yields of extracellular tannase under solid state fermentation ssf using different agro forest residues such as amla leaves phyllanthus emblica, ber leaves zyzyphus mauritiana. Bhaskar reddy and vandana rathod department of microbiology, gulbarga university, gulbarga 585 106. Solid state fermentation technology ssf easy biology class. Solid state fermentation for tannase production by fungal. The metabolic processes of microorganisms are influenced by changes in parameters like temperature, ph, incubation time, humidity etc. Tannase production by aspergillus fumigatus ma under solidstate. Production of tannase by various fungal cultures in solid. Production of tannase by citrobacter freundii under solid state fermentation and its application in fruit juices debittering s. Fermentation is the primary technique for the production of various enzymes. An autochthonous tannase yielding yeast strain pichia kudriavzevii gu939629, isolated from the gut of an indian major carp mrigal, cirrhinus cirrhosus, has been used for extracellular tannase production and subsequent processing of two plant feedstuffs, groundnut oil cake goc and pistia leaves pl, under solid state fermentation ssf.
Pdf production of tannase under solidstate fermentation. Mucor circinelloides, tannase, 18s rrna gene sequencing, sequential statistical strategy, solid state fermentation. Tannase production by aspergillus niger aa20 was studied in submerged smf and solidstate ssf fermentation systems with different tannic acid and. Mani 3 1 post graduate and research department of microbiology,dr. Tannase production by penicillium purpurogenum paf6 in. Three types of tannic acid media were used for submerged fermentation.
Among all agroindustrial waste material evaluated, cashew husk supported maximum tannase production. Novel optimization strategy for tannase production through a. Tannase production in solid state fermentation using groundnut shell tannase production by a. Ssf is the preferred method for tannase production because of its lower cost, lower water consumption, easier operation, and higher enzyme activity than slf 16, 17, 18. Both fungi and bacteria yield an invaluable array of enzymes when fermented on appropriate substrates. Crude tannase enzyme activity at various time intervals for each of the fungus was analyzed by spectrophotometric method. Optimization of tannase production by aspergillus niger in solid. Optimization of tannase production and improvement of. Production of tannase under solid state fermentation tea stalk powder 5 g was placed in 250ml erlenmeyer flasks and moistened with a mineral salt solution 0. Castor bean residue, a toxic residue and byproduct of brazilian biodiesel production, was inoculated with a strain of p.
The production medium is often simple, using agroindustrial byproducts like wheat bran, rice bran, or wheat straw as the substrate mitchell. The microbial fermentation increases the nutrient content and flavor of food products. Tannase is an industrially important enzyme produced by a large number of microorganisms. Pdf tannase enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves zyyphus. The industrial production of tannase can be performed through solid state fermentation ssf by the use of tannin rich fruit residues, in order to minimize production costs.
Arts and science college, coimbatore 641 035 india. The effect of initial water content was studied and maximum enzyme production was obtained when 60 ml of water was added to 100. A tannase yielding fungal culture identified as aspergillus fumigatus ma was isolated from the effluent collected from a local small scale tannery. Solid state fermentation ssf solid state fermentation ssf is a fermentation method used by several industries like the pharmaceuticals, food, textile etc. The tannase enzyme production was compared under submerged and solid state fermentation conditions using aspergillus niger. The fungal strain was able to grow on cab without any supplementation but a. Tannase production by solidstate fermentation ssf is more. Various low cost tannin rich residues such as coffee husk, tamarind seed powder, tea leaves and triphala powder were studied in semi solid state fermentation process. Tannase production by solid state fermentation of cashew. Industrially the production of tannase is usually performed via submerged fermentation sf. The ability of aspergillus oryzae for the production of tannase by solid state fermentation was investigated using cashew apple bagasse cab as substrate. Tannase is an important enzyme which finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid.
The optimum ph of the moistening agent was found to be 5. Extra cellular production of tannase by aspergillus foetidus, rhizopus oryzae pure culture and co culture under solidstate fermentation using tamarind seed powder was evaluated. An attempt has been made to optimize the production of enzyme tannase by solid state fermentation ssf using the organism rhizopus oryzae. Study is on the solid state fermentation of tannase with the gallnut by aspergillus niger b0201. Tannase production methods include solidstate fermentation ssf 11,12, and submerged liquid fermentation slf 11, 14, 15. Production of tannase from aspergillus ruber under solid. Research article purification and characterization of. In 96th hours all the organisms produced more tannase. Smf is usually implemented in case of bacterial enzyme production, due to the requirement. Tannase production by aspergillus niger aa20 was studied in submerged smf and solid state ssf fermentation systems with different tannic acid and glucose concentrations. A modified solid state fermentation was used to produce mevastatin by penicillium citrinum ncim 768 using wheat bran as carrier in glycerol and urea based medium as moistening agents. The best favourable conditions for enzyme production include initial ph 5 with 4 days of incubation period at 40c and 72% humidity, and 10 g wheat bran soaked in 2.
Production of mevastatin by solidstate fermentation using. Abstract the influence of various carbon sources and nitrogen sources on tannase and gallic acid production in ssf by p. Experimental design results 2 3 for tannase production at 96 h of ssf. The experiments involved in the process and conditions of solid state fermentation and factors influencing characters of tannase. Amount of 35 strains of fungi species isolated from soil of caatinga were used for qualitative selection of strains with potential for production of tannase. Production of tannase from aspergillus niger under solid. Jamun leaves were found to be the best substrate for enzyme production under solid state fermentation ssf. Research on conditions of solidstate fermentation and. It is defined as the growth of microbes without freeflowing aqueous phase. The production medium is often simple, using agroindustrial byproducts like wheat bran, rice bran, or wheat. Production of tannase under solid state fermentation tea stalk powder 5g wasplaced in 250mlerlenmeyer.
Production of tannase by aspergillus niger aa20 in submerged and solid state fermentation. Solidstate fermentation ssf is defined as the growth of microbes without free flowing aqueous phase. Tannase finds application in many industrial sectors which includes pharmaceutical, food, chemical and beverages industry. After cooling, the samples were inoculated with some. Solid state fermentation ssf was evaluated to produce tannase from aspergillus niger. The ssf is alternative to submerged fermentation for production. The highest yield of tannase was obtained at 96 hour 4th day incubation period, at 300c temperature and ph 5. Through the selected fungi, a complex study was achieved about the influence of the. Production of tannase from aspergillus ruber under solidstate. Production of tannase by aspergillus niger aa20 in submerged and. A promising method for production and optimization of tannase from bacillus gottheilii m2s2, abstract tannase is an important enzyme which finds commercial applications in food industry to reduce the level of tannins in fruit juices, preparation of instantaneous tea and production of gallic acid. Pdf production of tannase under solidstate fermentation using. Novel optimization strategy for tannase production through. Production of tannase from aspergillus ruber under solid state fermentation using jamun syzygium cumini leaves.
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